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resource source identifier xhoi neb cat  (New England Biolabs)


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    New England Biolabs resource source identifier xhoi neb cat
    Resource Source Identifier Xhoi Neb Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+xhoi+neb+cat/XhoI/pm41335518-223-2-6
    Average 99 stars, based on 9092 article reviews
    resource source identifier xhoi neb cat - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:


    Staining:


    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    Cell Stimulation:


    Adjuvant:


    Western Blot:


    Real-time Polymerase Chain Reaction:


    Reverse Transcription:


    Recombinant:


    Plasmid Preparation:


    Protein Extraction:


    RNA Extraction:


    Marker:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    Transfection:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    cDNA Synthesis:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    DNA Extraction:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    SYBR Green Assay:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    Gel Extraction:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    Protein Purification:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    Chromatin Immunoprecipitation:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    Immunoprecipitation:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    SDS Page:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..

    RNA Sequencing:

    Article Title: Multi-site phosphorylation of a transcription factor orchestrates imidacloprid resistance in whitefly.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit total anti-CYP306A1 HUAAN N/A Rabbit total anti-Eagle HUAAN N/A Rabbit total anti-p38 Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit total anti-ERK Yang et al. 8 https://doi.org/10.1073/pnas.1913603117 Rabbit phosphorylated anti-Eagle at S363 HUAAN N/A Rabbit phosphorylated anti-Eagle at Y235 HUAAN N/A Rabbit phosphorylated p38 MAPK CST Cat#9215S; RRID: AB_3317262 Rabbit phosphorylated anti-ERK CST Cat#4370S; RRID: AB_2315112 Anti-β-actin Abcam Cat#ab115777; RRID: AB_10899528 goat anti-rabbit lgR-HRP ABclonal Cat#CW0103S; RRID: AB_3251479 anti-6X His-Tag antibody Abcam Cat#ab9108; RRID: AB_307016 Goat anti-Mouse IgG Abcam Cat#Ab150113; RRID: AB_1050113 Bacterial and virus strains Escherichia coli BL21 cells Vazyme Cat#C504-03 Biological samples DNA of whitefly This study N/A RNA of whitefly This study N/A gDNA of whitefly This study N/A Total protein of the nymph whitefly This study N/A Chemicals, peptides, and recombinant proteins 5-Hydroxy imidacloprid TRC Cat#H943220 3-Hydroxy imidacloprid BESTECH Cat#C14283719 imidacloprid -urea Selleck Cat#S4448 6-Chloronicotinic acid First Standard Cat#09-C11452000 olefin imidacloprid First Standard Cat#1ST20548 imidacloprid -guanidine hydrochloride PANPHY Cat#FD019901 Desnitro imidacloprid PANPHY Cat#FD019901 imidacloprid Sigma-Aldrich Cat#37894-100MG Ferric citrate Sigma-Aldrich Cat#F3388-250 NADPH Regeneration System Promega Cat#V9510 H89 Selleck Cat#130964-39-5 DMSO Amresco Cat#0231-500ML IPTG Beyotime Cat#ST1416-5G BeyoGold TM His-tag Purification Resin Beyotime Cat#P2233-100mL Protease inhibitor Cocktail Medchemexpress Cat#HY-K0010 Lysozyme Beyotime Cat#ST206 Imidazole Beyotime Cat#ST1402-25G 4%–20% SDS-PAGE gel Lablead Biotech, China Cat#180-8001H 1 x PBS JSENB Cat#JF7384-24×500mL-VAR SD/-Leu with Agar Clontech Cat#630311 SD/-Ura with Agar Clontech Cat#630315 HindIII NEB Cat# R0104V BamH1 NEB Cat#R0136V (Continued on next page) 14 Cell Reports 44, 116638, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER XhoI NEB CatR0146S BstBI NEB Cat#R0519V Aureobasidin A Clontech Cat#630499 DTT Solarbio Cat#190228-30 ColorMixed Protein Marker 180 Abclonal Cat#RM19001 Nanomaterials China Agricultural University of Professor Jie Shen N/A Lipofectamine 2000 transfection reagent Thermo Fisher Scientific Cat#11668019 Critical commercial assays SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34577 PrimeScript II 1st strand cDNA synthesis kit TaKaRa Cat#6210B PrimeScript RT kit TaKaRa Cat#RR014A Trans1-T1 competent cells TransGen Cat#CD501-02 TIANamp Blood/Tissue/Cell Genomic DNA Extraction TIANGEN Cat#DP304-02 SuperReal PreMix Plus (SYBR Green) TIANGEN Cat#FP205-01 50 x ROX Reference Dye TIANGEN Cat#FP205-01 MiniBEST Universal Genome DNA extraction kit Takara Biotech Cat#9765 Monarch DNA Gel extraction kit NEB Cat#T1020 His-tag Protein Purification Kit Beyotime Cat#70502-K10 GST fusion protein purification kit BEAVER Cat#70601-K10 InstanBlue Coomassie Protein Stain Abcam Cat#Ab1192211 LightShift Chemiluminescent EMSA Kit ThermoFisher Scientific Cat#20148 EZ-Magna ChIP A/G Kit Merk Millipore Cat#17-10086 BeaverBeads TM Mag501 ProteinA/G Matrix Immunoprecipitation Kit Beyotime Cat#22202-100 Mn 2+ -Phos-tag TM SDS-PAGE kit Wako Cat#1301-93521 T7 Ribomax Express RNAi System Promega Cat#P1700 Dual-luciferase assay system Promega Cat#E1960 Bac-to-Bac Baculovirus Expression System Invitrogen Cat#10359016 10 x TBE Solarbio Cat#T1051 Deposited data Bemisia tabaci MEAM1 genome Chen et al. 62 http://www.whiteflygenomics.org/ cgi-bin/bta/index.cgi Bemisia tabaci MED genome Xie et al. 63 http://gigadb.org/Table.S4/100286 RNA-seq data for Bemisia tabaci MED Tian et al. 64 NCBI SRA: SRP064690 Experimental models: Cell lines Spodoptera frugiperda: cell line Sf9 Invitrogen Cat#B82501 Drosophila melanogaster: cell line S2 This study N/A Experimental models: Organisms/strains B. tabaci MED reared on cotton (Gossypium herbaceum L. cv. ..



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